https://t.me/DrAriyanaLove/26910
Dr. Ariyana Love, [Aug 21, 2023 at 13:10]
#EDTA IS A PRECURSOR TO CELLULAR TRANSFECTION WITH #modRNA.
“We found that chemically abrading the differentiated CACO-2 human intestinal epithelial cell layer by a trypsin and EDTA pretreatment (before the use of detergent-like transfection reagents) dramatically improved transfection efficiency in this polar, differentiated model. Although this treatment did improve the transfection efficiency, it also induced leakiness in the epithelial barrier by both opening tight junctional complexes and by creating holes in the cell layer because of low-level cell death and detachment. Thus, this approach to enhance the transfection efficiency of polar, differentiated cells will be useful for assessment of the effect of the transfected/expressed protein on (re)formation of an epithelial barrier..."
https://pubmed.ncbi.nlm.nih.gov/31037041/
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https://t.me/DrAriyanaLove/26911
Dr. Ariyana Love, [Aug 21, 2023 at 13:28]
THERMO FISCHERS FORMULA FOR CELLULAR TRANSFECTION USING #EDTA
“Formaldehyde solution, Thermo Scientific Shandon Immu-Mount, Hoechst 33 342 (1.0 mg mL−1 in water) and propidium iodide (PI, 1.0 mg mL−1 in water) were purchased from Thermo Fisher Scientific Inc. (MA, USA). ScreenFectA transfection reagent was obtained from ScreenFect (Eggenstein-Leopoldshafen, Germany). Fetal calf serum (FCS), RPMI-1640 cell culture medium, 0.25% trypsin/EDTA, Dulbecco's Modified Eagle's Medium (DMEM), 1% penicillin/streptomycin solution were purchased from Gibco, Life Technologies GmbH (Darmstadt, Germany). Ham's F12 medium was purchased from Biowest (Nuaillé, France)."
https://onlinelibrary.wiley.com/doi/full/10.1002/adbi.201900257
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https://t.me/DrAriyanaLove/26912
Dr. Ariyana Love, [Aug 21, 2023 at 13:52]
EDTA induces human cell death and cancer in response to an acidified extracellular environment.
EDTA induces human T-cell death-associated with gene 8 (TDAG8) by overexpressed HEK293T cells.
👉T-cells are the critical part of our immune system that stops foreign invaders from entering our cells. EDTA is essentially assisting the CONVID jabs to take out your immune system.
EDTA also induces ovarian cancer from the G-protein-coupled receptor 1 in human leukemia cell line HL-60.
https://www.sciencedirect.com/science/article/abs/pii/S0006291X22010968
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https://t.me/DrAriyanaLove/26914
Dr. Ariyana Love, [Aug 22, 2023 at 03:05]
Yes! I was CORRECT!
#EDTA is a genotoxin, meaning that it does in fact negatively alter your DNA. EDTA inhibits DNA synthesis by disrupting the natural process of DNA replication! EDTA causes morphological changes of chromatin, interfering in cell replication! EDTA induces gene mutations and induces chromosomal breakage, meaning that it will have a damaging effect on your offspring, effecting generations!
https://pubmed.ncbi.nlm.nih.gov/6406880/
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https://t.me/DrAriyanaLove/26918
Dr. Ariyana Love, [Aug 22, 2023 at 13:22]
EDTA is used to clean power plant boilers! As an acid (oxidant) it burns through everything.
https://www.researchgate.net/publication/245300037_Recovery_of_EDTA_from_Power_Plant_Boiler_Chemical_Cleaning_Wastewater
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https://t.me/DrAriyanaLove/26919
Dr. Ariyana Love, [Aug 22, 2023 at 13:38]
Here’s a very deceptive paper on EDTA that could easily mislead someone to think that EDTA is an “antioxidant”, based on the title.
In the Conclusion of this short-term study it states that “EDTA treatment induces the activity of antioxidant enzymes”.
The average person would read this and think something beneficial is occurring as a result of taking EDTA but that’s not actually what it’s saying.
Antioxidant enzymes are proteins involved in the catalytic transformation of reactive oxygen species. “Induces the activity” means that the Mitochondria are reacting to poisoning (ROS)!
So, very deceptive indeed! This is what I call word sorcery.
https://pubmed.ncbi.nlm.nih.gov/21722414/
https://t.me/DrAriyanaLove/26920
Dr. Ariyana Love, [Aug 22, 2023 at 13:49]
SIDE EFFECTS OF EDTA
👉Kidney or renal failure
👉Prolonged bleeding
👉Tetanic seizure
👉Arrhythmia
👉Hypocalcemia
👉Hypotension
Other symptoms may include:
Muscle aches
Chills
Fever
Headache
Nausea or vomiting
Dark or cloudy urine, or trouble urinating
Numbness and tingling
Fatigue
Racing heart beat
Allergic reactions like hives, swelling, itching or rash
https://t.me/DrAriyanaLove/26921
Dr. Ariyana Love, [Aug 22, 2023 at 13:58]
Oral exposures to EDTA produced adverse reproductive and developmental effects in animals.
https://pubmed.ncbi.nlm.nih.gov/12396676/
https://t.me/DrAriyanaLove/26922
Dr. Ariyana Love, [Aug 22, 2023 at 14:07]
The toxic effects of EDTA included breast-milk cell loss, disruption of milk fat globule membrane and subsequent release of membrane-bound protein, free fatty acids and reduction in pH. It also caused false-positive results of haemolytic assays (loss of red blood cells).
https://pubmed.ncbi.nlm.nih.gov/10547620/
https://t.me/DrAriyanaLove/26923
Dr. Ariyana Love, [Aug 22, 2023 at 14:14]
EDTA CHELATION THERAPY
EDTA is poorly absorbed from the GI tract (<5%) and, as a consequence, should only be administered by a parenteral route. It is primarily distributed in extracellular fluids, which limit its capacity to chelate intracellular metals. EDTA is also known to redistribute lead to the brain and should not be used to assess for lead mobilization in children. EDTA can be obtained as disodium EDTA or calcium disodium EDTA with different applications of use. The FDA archived a report from 2013 entitled, “Questions and Answers on Edetate Disodium (marketed as Endrate and generic products.” EDTA is used to refer to two similar but separate drugs (pharmaceuticals) with differing indications of use. The first drug is calcium disodium EDTA (Versinate), and the second drug is disodium EDTA (Endrate). The confusion lies in the common name of EDTA for both drugs; however, there are two very separate indications of use for each. Calcium disodium EDTA is approved by the FDA for use in lead poisoning and has been the mainstay of treatment for childhood lead poisoning since the 1950s. The second drug, disodium EDTA, is approved for use in patients with rhythm disorders from drug intoxication such as digitalis where there is hypercalcemia. Calcium disodium EDTA, Versinate, will not deplete calcium if given rapidly, while disodium EDTA will remove calcium through renal excretion in a life-threatening fashion if administered rapidly. In fact, erroneous administration of a bolus infusion of disodium EDTA for lead poisoning, when calcium disodium EDTA was meant to be administered, resulted in the death of a child. Between 1971 and 2007, there were 11 deaths associated with the use of EDTA infusions. Nine of these deaths were attributed to disodium EDTA specifically, while two of the deaths only refer to EDTA as the causative agent. In fact, in five of the deaths, there was confusion when ordering the drug that resulted in the adverse outcome. There have been no deaths reported from administering calcium disodium EDTA. Risks associated with IV EDTA chelation have been reported to be renal failure, arrhythmia, tetany, hypocalcemia, hypotension, and prolongation of bleeding time, among others.
— Jeanne A. Drisko MD, in Integrative Medicine (Fourth Edition), 2018
https://t.me/DrAriyanaLove/26924
Dr. Ariyana Love, [Aug 22, 2023 at 14:39]
Because of its adverse effects, calcium EDTA is being progressively replaced by DMSA in the treatment of lead poisoning. The calcium chelate of EDTA (CaEDTA) has shown teratogenic (malformation) effects and produced abnormalities in rat pups. The malformations consisted of submucus cleft, cleft palate, adactyly/syndactyly, curly tail, and abnormal ribs and vertebrae.
~Handbook on the Toxicology of Metals
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Dr. Ariyana Love, [Aug 22, 2023 at 14:56]
This document from the United States Environmental Protection Agency refers to EDTA as "ethylenediaminetetraacetic acid (EDTA)". It is in fact an "acid" and therefore EDTA is a an oxidant, not an antioxidant.
"Its ability to bind heavy metal ions can be used to sequester these trace metals. However, trace amounts of various metals are necessary for the proper functioning of the body. If instead, these minerals were bound to the EDTA, then deficiencies of these trace metals could result.
In fact, the toxic effects of EDTA are considered to be related to metal deficiencies, especially a deficiency of zinc."
This document also refers to two studies done on rats where maternal and developmental "effects" resulted. We know that means defects.
https://www.epa.gov/sites/default/files/2015-04/documents/edta.pdf
https://t.me/DrAriyanaLove/26926
Dr. Ariyana Love, [Aug 22, 2023 at 15:12]
EDTA AN ENVIRONMENTAL POLLUTANT
EDTA negatively impacts the metal balance in natural waters as well as in aquatic organisms, even in the long term.
“EDTA contribute to the aquatic toxicity at significantly lower concentration than those determined by short-term toxicity tests", meaning that only small amounts are needed to negatively impact our environment in the long-term.
👉As a general rule, if it’s not good for the environment what makes you think it’s good for you??
https://pubmed.ncbi.nlm.nih.gov/9297986/
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